Well, he appears to be somewhat intelligent, so I would say: yes, most likely.

Why does it matter if he's an atheist or not?
[Formerly CosmosX9]

Connsidering he says "F'ing Jesus" and such every video, I highly doubt he is Christian.

Are are you retarded or something?

Personally, I don't care if he's irreligious or not. He obviously isn't obsessed with the issue of religion, nor does he hold religious people in contempt. He just wants to make comedy. He's good in my book (I'm a Christian).

That twitter you quoted is not his wife. There is more than one April Rolfe in the world.

Trying to bring this back on topic
He does say some pretty sacrilegious things in his Bible games review. And I don't think a Christian would come up with Super Mecha Death Christ. But we can't be sure because he has never publicly stated one way or the other.
The best clue we have is actually from his wife's twitter account, where she describes herself as a "Wine & Spirit Importer, Blogger, Wife, Sister, Daughter,
Atheist
, Lover of all things Sci Fi & Sport, Wine Devotee, Fashion Enthusiast, Violet Ashes 5b4Gardener"
So we know for a fact his wife is an Atheist. That probably means James is either an Agnostic or Atheist, or at the least non-religious. It's unlikely someone who is very religious would marry an Atheist.

And Buybul is undoubtedly 100% truth and accurate According to classical christian apologist fallacy "you can't prove there's no gawd", you can't argue evolution is false either. If your bearded bloodthirsty space monster is "probable" and "scientifically relevant" "theory", then anything goes. And if take more critical aspect, then you can kiss goodbye to your bronze age mythologies BEFORE we start

Ok, then please explain to me how the first living cell popped into existence.
and yeah, i've read that whole evolution article on wikipedia, which is a pro-evolutionist web site anyway. But even they won't censor or ban an article unless it directly threatens their dogma of evolution. However, it's not very hard to put 2 + 2 together from the facts that are found even on their site. Just read their article on how DNA works - which is present even in bacteria, like e.coli - one of the simplest life forms known to exist.
btw, take a look at these:
https://www.youtube.com/watch?v=ApJ-nvNoTSg
https://www.youtube.com/watch?v=sYSRRBp2Wfg
and especially (rather funny):
https://www.youtube.com/watch?v=Ckfrn5-86xU

I'm not even going to reply to this because of the ridiculousness. I suggest for you to read this link:

If anything, evolution has been more or less proven to be false. If it was a fact, we'd have fossil records of "missing links" between every species and the next. For example, if the giraffe was really a descendant of the antilope, we would have fossils of intermediate forms with progressively higher necks. There are none.
Evolutionists concentrate on the "missing link" between apes and humans, when all they have is a handful of deformed specimens, which were probably diseased individuals to begin with. If these "ape-men" were more "fit" to survive than apes, then how come there are still apes, and not a single one of these ape-men?
Also, how did the first living cell come into existence? Even the simplest cell, such as the e.coli bacteria, is
extremely
complex. Next to it, the most advanced man-made computer is nothing but sticks and stones. Just look up how DNA works, which is present even in bacteria - a very complicated and powerful mechanism.
That's just a few of the serious flaws in the theory of evolution. Also, if it's a proven fact, why do evolutionists still disagree about fundamental aspects of it?

Achilles' reply is obviously a troll, but I'm still going to reply for beep and giggles. I suggest you turn off your browser right now, before I carefully explain how much of a beep idiot you are.
"Atheists have small dicks, low iqs, and ugly vaginas."
Very funny. As for the IQ part, at least we don't believe creationism even though evolution has been proven true decades ago.
"They like to blame god for not being perfect."
Isn't the whole point of atheists that they do not believe in any gods?
"Atheists and Liberals go together like glue."
This comment makes no sense whatsoever. Liberal, in other words, got its bad name because of a series of racial, sexual, and global bogeymen that dont frighten Americans nearly as much anymore.
"You beep ruined our Country and you're the reason why Murderers exist."
On the contrary. How come we ruined your country? We haven't done anything to wrong you. And as for the murdering part, isn't the largest reason for all the wars in the history of humanity religion? And aren't almost all of those mentally ill murderers thinking they are doing their things for god? Also, many inventors and engineers that have built devices and inventions you're using were atheists.
The rest of your message is filled with insulting atheists without a reason, prejudice and threatening. I thought Jesus said in his teachings that you should love others like he loved you, take care of them and that you shouldn't judge them, even if they are gay, worship other gods or don't worship any god. If you think you're going to heaven with your behavior, I have bad news for you.
And more than ever, this video is relevant: https://www.youtube.com/watch?v=5hfYJsQAhl0

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"Most Atheists I know don't have children and are deadbeats."
Are you serious? Just because you don't share the same lifestance or beliefs doesn't make anybody "a deadbeat". It only makes you stupid, because you judge people according to their views. Doesn't your religion count that as a sin? You're pathetic. Atheists aren't any different from other people. All atheists that I know have a normal life and family. You are the "deadbeat" here making these false acclaims. Also FYI, atheists celebrate Christmas too, including me, we are in the same culture after all, and atheist often get married too.
This is for you, "Achilles": https://www.youtube.com/watch?v=5hfYJsQAhl0

Most Atheists I know don't have children and are deadbeats. That fits the description of Archfiend. That slimy internet creepy guy that always attacks James Rolfe. If I had to guess, James Rolfe is probably Christian or Catholic since he celebrates Christmas and Rol2000fe has never said anything bad about Religion. Unlike Archfiend who likes to attack people that are actually successful at life.

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That video did disappoint me if I'm going to be totally honest, but James jokingly replied to someone pointing it out with:
Well on the last episode of Star Trek: The Next Generation Q said humans came from goo. I'll just go with whatever Q said
Honestly, I think they're just scientifically illiterate, as many atheists are, and as some religious people aren't. They probably don't care. I'd guess most people just don't care.
Or they are religious. That's possible, but they never did strike me as religious fellas. Then again, Kevin Smith is a believer - devout even, from I understanding
Sad story. You got a smoke?

In an episode of 'James & Mike Mondays' they discuss evolution and ghosts. It seems they aren't familiar with evolution, so I don't think he or Mike are atheists.
Here's the link, the episode is 'Action Max'.
https://www.youtube.com/watch?v=mj498-wkOz4
Also, who cares? I don't care about anybody's religious views, and neither should you.