Love the opening to CR - the bathroom fight and his visit to his man in Prague.
The opera scene in QOS, especially the stand off and glare at the bottom of the staircase.

Another one of my favourites:
M:
l'm relieving you from Operation Bedlam, 007.
Bond:
But, sir, Blofeld's something of a must with me.
M:
You've had two years to run him down.
Bond:
Have you lost confidence in me?
M:
I'm well aware of your talents, but a licence to kill is useless unless one can set up the target.
Bond:
Sir
M:
I'II find you a more suitable assignment.
Bond:
Under the circumstances
M:
That's all!
Moneypeeny:
That was a quick conference. How can a girl keep herself alluring
Bond:
Take a memo, please, Moneypenny.
Moneypenny:
Ready, James.
Bond:
Sir, I have the honour to request. if you'II accept my resignation effective forthwith.
Moneypeeny:
Resignation from what?
Bond:
Her Majesty´s Secret Service. And kindly present it to that monument in there.
(door opens and slams shut)
(intercom)
Bond:
James Bond here.
Moneypenny:
He wants you, James.
M:
Request granted.
Bond:
Request granted. Not even with regret.
Moneypenny:
What did you expect? A knighthood? Why don't you read it?
Bond:
Two weeks' leave!
Moneypenny:
Well, you didn´t really want to resign, did you?
Bond:
Moneypenny, what would I do without you?
My DVD Collection:
http://www.imdb.com/list/ls063878029/

very dififuclt to pick just one, but probably the scene where Bond escapes from the crocodiles in Live And Let Die.

the pretitties are plentyful in the beginning of the Bondfilms!

Verily I say unto theegotta love those Pre tittles sequences"

The Bliibao, bank scene- The World is not enough
The love scene with Paris- Tomorrow never dies
Swordfight between Bond and Gustave Graves- Die Another day
James Bond introduction at the Casino- Dr No
I decdied to accept your invitation- Dr No
Bond Meets Vesper - Casino Royale
Bond meets Severine- Skyfall
Pre tittles sequence- Skyfall

Verily I say unto thee the scene on the stealth ship during the climax of TND when Bond kicks a dead or dying guard in the head just for the sake of doing so (pleasing sound effect too). Great stuff and just what a vengeful naval commander would do in such circumstances.

Leonid Pushkin:
I take this is not a social call, 007.
James Bond:
Correct.
James Bond:
You should have brought lilies.
Leonid Pushkin:
May I ask why?
James Bond:
Smiert Spionam.
Leonid Pushkin:
Smiert Spionam? Was a Beria operation, in Stalin's time. It was deactivated 20 years ago.
James Bond:
Two of our men are dead.
Leonid Pushkin:
My condolences. We had nothing to do with it.
James Bond:
Get down on your knees. Put your hands behind your back.
Leonid Pushkin:
You are professional. You do not kill without reason.
James Bond:
Two of our men are dead. Koskov's named you. Why should I disobey my orders?
Leonid Pushkin:
I am in the dark as much as you. It is a question of trust. Who do you believe? Koskov, or me?
James Bond:
If I trusted Koskov we wouldn't be talking. But as long as you're alive, we'll never know what he's up to.
Leonid Pushkin:
Then I must die.

Bernard Lee's delivery of "Operation Bedlam is dead" definitely sticks in my head. Heard it in his voice right when I read the phrase.
For me it's a tough call between Bond vs Grant and the endings of both OHMSS and CR.

Thy dawn, O Master of the World, thy dawn;
For thee the sunlight creeps across the lawn,
For thee the ships are drawn down to the waves,
For thee the markets throng with myriad slaves,
For thee the hammer on the anvil rings,
For thee the poet of beguilement sings.