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I myself have never heard the reference to Agnes Moorhead, but somehow I doubt it. For starters, while Moorhead's sexuality was a matter of speculation for years, she did have two husbands along the way, for whatever that was worth. As for her behavior, Moorhead's reputation was as a consummate professional who would never have lowered herself to the antics that June Buckridge gets into here.
I remember reading somewhere that Moorhead was one of the best-liked performers in Hollywood; it is doubtful that anyone would have said that about her if she were anything like June Buckridge, whatever her sexuality may have been.
Never mess with a middle-aged, Bipolar queen with AIDS and an attitude problem!

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A much more likely model is the British journalist Nancy Spain.It was well known that she was a lesbian (according to Wikipedia she was a regular at the Gateways club)and she frequently appeared on the radio in panel games alongside Lady Isobel Barnet, a very refined lady, who may well have been the model dfor Mercy.