There are too many scenes to mention. But I would like to mention several of my choices:

• the opening montage in its entirety is such a beautiful and powerful evocation of the experiences of Union soldiers in camp, marching, trudging through rain, passing escaped slaves and going into the battle. It builds up to a climax when depicting the scenes at Antietam. The whole is about as close to cinematic poetry as I have ever seen.
• when the troops come to Beaufort South Carolina and Morgan Freeman talks to a bunch of young (former) slaves. He says 'that's right hon, we're runaway slaves, come back as fighting men' What an accomplishment

OMG, so many favorites!
The lovely call and response scene under the tree at night, with the preaching by the stammerer and by Morgan.
Morgan slapping Trip in the face and telling him some home truths about what a bullying jerk Trip was.
The quartermaster scene ("You nasty little cuss").
I choked up when the same guys who had a fight with Trip earlier, saw them passing into the defile, and the main white guy shouted "Give 'em Hell, 54th".

Everything you all said
Including the scene when Sgt Maj Mulcahy salutes the troops during the parade. Shows that he was incredibly hard on them, but proud of the turn out.
"We grew up together"
-"Then let him grow some more."
Look not into the eyes of the hungry, for despair is a contagious disease.

• Gary Anthony Williams

Another good scene that required no dialogueColonel Shaw looking out to sea before the final battle as if taking it all in one last time. When he released his horse, you knew he didn't plan on surviving the assault. Makes my eyeballs sweaty every-time.

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I agree with what everyone else have already said.
Like to also add the scene where they finally get their blue uniform.
Favourite scenes though:

1. Shaw looking out to sea
2. whipping scene
3. Rawlins slaps Trip

Many good scenes already mentioned. I like right before the final battle, where Shaw reviews his men. No words, just the music and his approving smile. It says so much about all they did to get to this point.
And in that scene, I like the last exchange between Thomas and Robert. He says, "I'll see you in the fort, Thomas." To me, it says, "I know you've grown up. We both have. But you're still my friend."

That paycheck tearing scene, pay refusal was a good scene. Showed Colonel Shaw not only talked the talk, but walked the walk with his men.

Also the scene where Col Shaw tears up his check with his men.

Every scene mentioned above me
One scene that still strikes me. Is when Pvt. Jupiter Sharts is showing off his shooting skills to the platoon at the range. And Col Shaw teaches the men a very important lesson about the stresses of being on the battlefield.
This is one of the most moving movies I have ever seen. I remember every second of watching this movie in 6th grade.

I forgot to mention the scene where Thomas saves Trip's life. The look on Trip's face when he realises that the man he has mocked and belittled as house n****r saved him from certain death.

The Battle of Ft Wagner itself, awed me when I first saw it. And still does.

I was really moved in the scene showing the conversation between Col Shaw and Sgt Maj Mulcahy regarding the drill sergeant being 'hard' on Thomas during training. I just loved the Sergeant Major's reply to Col. Shaw.
Sgt Major Mulcahy:"You grew up with the boy?"
Col. Shaw : "Yes"
Sgt Major Mulcahy: "Well let him grow up some moresir"

That was a moving scene, especially the sermon given by Jupiter Sharts.

Excellent collection of memorable scene. I would like to add that night when they were singing before last war and Trip told the unit that he had no family but this is his family.

I can't remember when I last teared up in a movie but I did for
Glory
. Thanks to actress, Diahann Carroll, for her request to show it on TCM.
Denzil Washington was sensational and earned the Oscar for his first co-starring role.
katie keene

I just came here because of the "give em hell 54th" line brought tears to my eyes just now. Glad to see it moved others as well. Powerful scene.
Haters gonna hate

After shaw dies
and drops the flag and then Trip picks it up, even after he said he didn't want to carry the flag because he was fighting for himself and not the US. That gets me every time.

The whole build up to the charge on Fort Wagner. Everyone knows it's a suicide mission, yet they're still willing to sacrifice their lives for their liberty. It's one of the most powerful and moving sequences I've ever seen. And it gets me every time.

The scenes you mentioned were indeed great and memorable, and here are some add-ons:

• Trip's confrontation with Thomas in the tent after the latter was beaten by the drill sergeant. Trip says to him, "You learnin' now, ain't ya Snowflake?" (Translation: You thought that because you're well educated and speak the King's language white folks will accept you as their equal, but it doesn't work like that.)
• When Trip agitates Thomas to the point that Thomas finally decides to fight him, Rawlingswho was just appointed Sergeant Majorintervenes and berates Trip for his hatred of everything and everyone. It's then that Trip realizes that his own self-hatred, which happens to be his worst hatred, is leading to his self-destruction. (Why Freeman wasn't nominated for an Oscar for his brilliant, powerful performance in this film was, IMO, a crime.)
• After Shaw is shot during the attack on Ft Wagner, Trip, who initially declined to carry the union flag, not only takes the U.S flag but assumes the leadership of the 54th by screaming to the top of his lungs "CHAAARGE!!" The scene, which I thought was the most powerful one in this film, powerfully portrays the immense courage of the 54th as well as pays homage to the black soldiers of the Union who, as Lincoln himself said, "help turn the tide of the war."

All of the examples given are excellent, but one not mentioned is the scene where Col. Shaw announces to the troops that the Confederate government had decreed that any black soldiers captured in Union uniform would be summarily shot, as would any white officers captured leading black troops. Shaw says that anyone wishing to be discharged would be allowed to do so. This took place at night in the rain. In the morning, Shaw emerges and asks how many are left. He then looks out and all are there, in formation. Shaw says, "Glory Hallelujah!". Gives me chills every time.

All great scenes. But for some reason I also find the drummer boys scene moving. Where they reluctantly leave before the attack and the Sergeant-Major says something like "Get along child, we'll be by directly." You know he doesn't believe it.
And I still tear up when Tripp's lifeless body rolls onto Colonel Shaw's.

Number one is my favorite:
Colonel Shaw, a boy from Massachusetts, looks out on the Atlantic Ocean peacefully for what is likely the last time in his young life. He looks over his shoulder at the ominous Fort Wagner knowing that is going to be the place of his death. And his face shows that he's afraid. Yet, you can see him accepting his fate as he dismounts and slaps his horse away. That's courage!
And then walks in among his men who take-up a cheer for him. The look of sheer pride on his face as he walks in amongst this extremely well-trained and motivated regiment, who chant "Colonel Shaw!" in appreciation for taking his job seriously and turning them into good soldiers, is a sight to behold. (Matthew Broderick did such an amazing job with this role!)

My favorite scenes:

1. GIVE 'EM HELL '54.
2. Morgan Freeman telling Trip off.
3. The black kids waving to the troops as they marched through town.
4. Trip going uphill saying "Come 'on".
5. Trip raising hell about the pay discrepancy between black and white soldiers.
6. Marching on the beach, like a ballet.
7. The 54 getting their new shoes and immediately marching.